Composite

Part:BBa_K3729003:Design

Designed by: Austin Wang, Julie Lin, Sinswien Lee   Group: iGEM21_KCIS_NewTaipei   (2021-10-09)


Strong promoter + strong rbs + BCD 1 gene + double terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 113
    Illegal SpeI site found at 230
    Illegal SpeI site found at 1312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 113
    Illegal NheI site found at 13
    Illegal NheI site found at 36
    Illegal SpeI site found at 230
    Illegal SpeI site found at 1312
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 113
    Illegal BamHI site found at 684
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 113
    Illegal SpeI site found at 230
    Illegal SpeI site found at 1312
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 113
    Illegal SpeI site found at 230
    Illegal SpeI site found at 1312
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References